Organizations are invited to share data or materials for inclusion in the Nextstrain build, which can be accessed at. Sharing data with this interactive online tool will enable the plant virology field to better understand and communicate the diversity and spread of this new virus. This paper describes a Nextstrain build containing ToBRFV genomes up to and including November 2019. No correlation was found between genotypes, companies and epidemiological traits, and the source(s) of the Dutch ToBRFV outbreak remain unknown. Take a look at your work habits on the stats page and sign in to sync your data. The 50 ToBRFV genomes from these outbreak locations group in three main clusters, which are hypothesized to represent three original sources. Pomodor is a pomodoro timer web app based on The Pomodoro Technique, made to help you focus on what matters It allows you to break down your work into sessions, label them, and get a notification when the time is up. By November 2019, 68 companies had been visited of which 17 companies were found to be infected. Nextstrain was used to visualize these potential connections. A follow-up survey was initiated to determine the extent of ToBRFV presence in the Dutch tomato horticulture and identify possible linkages between ToBRFV genotypes, companies and epidemiological traits. Presence of the virus was verified using Illumina sequencing.
In October 2019, the Dutch National Plant Protection Organization received a ToBRFV infected tomato sample as part of a generic survey targeting tomato pests. Since the first description, the virus has been reported from all continents except Oceania and Antarctica, and has been found infecting both tomato and pepper crops. Tomato brown rugose fruit virus (ToBRFV) is a Tobamovirus that was first observed in 20 on tomato plants in Israel and Jordan respectively.
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